2010 IR Workshop

Hoi Ying Holman

[Click here for the talk abstract]


Hoi YIng Holman is part of the Structural Biology (BSISB) Program at the Advanced Light Source (ALS), the Head of Chemical Ecology in the Earth Sciences Division (ESD), and a Virtual Institute for Microbial Stress and Survival (VIMSS) Investigator. Holman’s research focuses on the development and application of technology to study the chemistry of living cells by integrating spectroscopy, microscopy, and microfluidics. She received her Ph.D. from University of California at Berkeley.


Real-time Monitoring of Chemistry in Living Cells During Stress-adaptive Response

Much of the current understanding of chemical reactions within cells has been derived from traditional biochemistry experiments that are either averaged over large populations or performed in vitro with purified biomolecules. However, these experiments generally cannot differentiate disparate phenotypes that can exist within a genetically homogeneous population of bacteria. For decades, infrared spectroscopy using a thermal emission infrared light source and an infrared microscope has been used to obtain detailed molecular information in biological specimens.

In this presentation, we will describe the advances that now allow IR spectromicroscopy to address this challenge and track biochemical reactions in situ in real time. We will highlight the role of the  synchrotron light source, which enables high-throughput noninvasive spectroscopic microanalysis and can precisely target subpopulations with diffraction limited spatial resolution and accuracy.